Gulfport ( GUHLF-port) is a city in Harrison County, Mississippi, United States, and its co-county seat. It had a population of 72,926 at the 2020 census, making it the second-most populous city in Mississippi, after Jackson. The Gulfport–Biloxi metropolitan area had a population of 416,259. Gulfport lies along the Gulf Coast of the United States in southern Mississippi, taking its name from its port on the Gulf Coast on the Mississippi Sound.

Gulfport emerged from two earlier settlements, Mississippi City and Handsboro. Founded in 1887 by William H. Hardy as a terminus for the Gulf and Ship Island Railroad, the city was further developed by Philadelphia oil tycoon Joseph T. Jones, who funded the railroad, harbor, and channel dredging. The city was officially incorporated in 1898. By the early 20th century, Gulfport had become the largest lumber export city in the United States, though this faded with the depletion of Mississippi's Piney Woods. The city transitioned into tourism through its white beaches, grand hotels, and significant casino gaming operations.

The largest sectors of Gulfport's economy include military operations, tourism, healthcare, and maritime commerce. The city is home to the Naval Construction Battalion Center, Gulfport Combat Readiness Training Center, and Mississippi Gulf Coast Community College. The Port of Gulfport serves as one of the busiest ports in the Gulf of Mexico, while Gulfport-Biloxi International Airport provides commercial air service to the region. Despite significant impacts from Hurricane Camille in 1969 and Hurricane Katrina in 2005, the city has consistently rebuilt and expanded its infrastructure and facilities.